SOLIScript® Lyo-ready 1-step RT-qPCR Kit
Description
SOLIScript® Lyo-ready 1-step RT-qPCR Kit is an optimized solution for fast and sensitive amplification and quantitation of RNA and DNA targets with probe-based assays. With a high collapse temperature (Tc) of -30 ˚C, the kit allows for the adoption of a rapid and energy-efficient lyophilization protocol. With a glass transition temperature (Tg) of 85.9˚C, the lyophilizates have a high resistance against long term exposure to higher temperatures. The composition is optimized for lyophilization to cakes or beads. The kit comes in 4 tubes: 40x SOLIScript® Lyo-compatible One-Step Mix, HOT SolisFAST® Glycerol-Free DNA Polymerase (10 U/μl), 4x Lyo Reaction Buffer and 4x RT-qPCR Lyo Excipient Mix.
40x SOLIScript® Lyo-compatible One-Step Mix is a 40x concentrated RT mix that includes an in silico designed SOLIScript® reverse transcriptase and RiboGrip® RNase Inhibitor. SOLIScript® is a thermostable reverse transcriptase active at temperatures up to 60°C, beneficial when using templates with high levels of secondary structure. RiboGrip® inactivates RNase A to protect RNA sample from degradation and increase cDNA yield in reactions with low RNA amounts.
HOT SolisFAST® Glycerol-Free DNA Polymerase (10 U/μl) is an in silico designed inhibitor tolerant analogue of Taq DNA polymerase with chemical hot-start and ~2-4 times faster extension rates compared to the wild-type Taq.
4x Lyo Reaction Buffer contains additives for efficient amplification. The MgCl2 concentration is 20 mM (1x mix – 5 mM).
4x RT-qPCR Lyo Excipient Mix is a 4x concentrated blend of lyophilization additives that protect reagents during freeze-drying and stabilize the lyophilizates. The mix enables lyophilization into both cakes and beads.
Applications
- Viral load determination in human diagnostics, veterinary and agricultural testing
- Pathogen detection
- Detection and quantification of RNA and DNA targets
- Lyophilization to beads and cakes
- High-throughput assays (fast cycling and reconstitution)
- Singleplexing and multiplexing (up to 5 plex)
Properties
Sample type: RNA and DNA
Hot start: Yes, chemical (10-minute initial activation at 95 °C)
Product format: Kit
Cycling speed: fast
Targets: 1-5
Reference dye: None
