HOT FIREPol® SolisGreen qPCR Mix 2.0

Description

HOT FIREPol® SolisGreen® qPCR Mix 2.0 is a 5x-concentrated solution specially designed for dye-based real-time quantitative PCR (qPCR) assays. SolisGreen® dsDNA binding dye is incorporated into the product to evaluate the DNA amplification process during PCR. The fluorescence spectra of this dye are similar to the more widely used SYBR® Green I and is compatible with most major real-time cyclers. When compared to SYBR® Green I, SolisGreen® dye shows:

• higher fluorescence level
• better sensitivity for detecting low template concentrations
• great stability for room temperature storage

HOT FIREPol® SolisGreen® qPCR Mix 2.0 is an optimized ready-to-use solution that contains all the necessary components, except sample (DNA template) and primers, to perform reactions with accurate and highly sensitive results. Additionally, the product includes a passive reference based on ROX dye making it compatible with both ROX-dependent and ROX-independent qPCR cyclers.

The product contains HOT FIREPol® DNA Polymerase to prevent the extension of non-specifically annealed primers and primer-dimers formed at low temperatures during qPCR setup.

Applications

• Gene expression analysis and absolute quantification
• Pathogen detection and quantification
• Low-copy gene detection
• Cell-free DNA analysis

For RNA applications try out an already optimized easy-to-use SOLIScript® 1-step SolisGreen® Kit 2.0 or combine HOT FIREPol® SolisGreen® qPCR Mix 2.0 with thermostable reverse transcriptases, such as FIREScript® and SOLIScript®!