6x DNA Loading Dye Buffer Double Blue
Description
6x DNA Loading Dye Buffer Double Blue can be used for loading DNA samples (PCR products, restriction fragments) on agarose or polyacrylamide gel.
The loading dye serves as a visual marker of electrophoresis: In 1% agarose gel Bromophenol blue comigrates with ca 300 bp (1 x TBE) or ca 400 bp (1x TAE) fragments and Xylene Cyanole FF comigrates with ca 3500 bp (1x TBE) or ca 5000 bp (1x TAE) fragments.
Properties
Reagent Composition: Bromphenol blue, Xylene Cyanole FF, TRIS-HCl, EDTA and Ficoll
Features: No interaction with the borate on TBE gels is observed, altering local pH due to the Ficoll component
Recommendations: Mix 1 volume of double blue Dye with 5 volumes of your DNA sample and load it into the gel
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